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In vivo treatment schedule and therapeutic outcomes. (A) Experimental timeline for control, radiotherapy (IR), <t>anti-PD-L1</t> (ICI), and combination groups. (B) Positioning of tumor-bearing mice for 6 MV x-ray irradiation. (C) Representative image of tumor harvesting on day 7. (D) Tumor-growth curves (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. ∗, ∗∗, ∗∗∗, or ∗∗∗∗ represent p-values <0.05, < 0.01, < 0.001, and <0.0001, respectively. X-axis: Number of days, corresponding to the treatment timeline, with the first day being the first day of treatment. Y-axis: Tumor volume growth, measured as the difference between the tumor volume on the day of measurement and the tumor volume before treatment (first day). (E) Body-weight changes over the study period (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. X-axis: Number of days. Y-axis: Mouse weight, measured as mouse weight on the day of measurement.
Anti Mouse Pd L1 Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vivo treatment schedule and therapeutic outcomes. (A) Experimental timeline for control, radiotherapy (IR), <t>anti-PD-L1</t> (ICI), and combination groups. (B) Positioning of tumor-bearing mice for 6 MV x-ray irradiation. (C) Representative image of tumor harvesting on day 7. (D) Tumor-growth curves (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. ∗, ∗∗, ∗∗∗, or ∗∗∗∗ represent p-values <0.05, < 0.01, < 0.001, and <0.0001, respectively. X-axis: Number of days, corresponding to the treatment timeline, with the first day being the first day of treatment. Y-axis: Tumor volume growth, measured as the difference between the tumor volume on the day of measurement and the tumor volume before treatment (first day). (E) Body-weight changes over the study period (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. X-axis: Number of days. Y-axis: Mouse weight, measured as mouse weight on the day of measurement.
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In vivo treatment schedule and therapeutic outcomes. (A) Experimental timeline for control, radiotherapy (IR), <t>anti-PD-L1</t> (ICI), and combination groups. (B) Positioning of tumor-bearing mice for 6 MV x-ray irradiation. (C) Representative image of tumor harvesting on day 7. (D) Tumor-growth curves (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. ∗, ∗∗, ∗∗∗, or ∗∗∗∗ represent p-values <0.05, < 0.01, < 0.001, and <0.0001, respectively. X-axis: Number of days, corresponding to the treatment timeline, with the first day being the first day of treatment. Y-axis: Tumor volume growth, measured as the difference between the tumor volume on the day of measurement and the tumor volume before treatment (first day). (E) Body-weight changes over the study period (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. X-axis: Number of days. Y-axis: Mouse weight, measured as mouse weight on the day of measurement.
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STAT1 inhibition assay. (A-B) Western blots and Annexin V/PI staining demonstrated that pharmacologic inhibition of STAT1 by <t>Itacitinib</t> abolished IFN-γ induced caspase-3 cleavage, and apoptosis in CT26.WT cells. Grayscale densitometry for all Western blots has been performed using ImageJ, normalized to β-actin (n = 3 independent experiments). Bars represent mean ± SD. Statistical comparison was performed using an unpaired two-tailed t-test.
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STAT1 inhibition assay. (A-B) Western blots and Annexin V/PI staining demonstrated that pharmacologic inhibition of STAT1 by <t>Itacitinib</t> abolished IFN-γ induced caspase-3 cleavage, and apoptosis in CT26.WT cells. Grayscale densitometry for all Western blots has been performed using ImageJ, normalized to β-actin (n = 3 independent experiments). Bars represent mean ± SD. Statistical comparison was performed using an unpaired two-tailed t-test.
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In vivo treatment schedule and therapeutic outcomes. (A) Experimental timeline for control, radiotherapy (IR), anti-PD-L1 (ICI), and combination groups. (B) Positioning of tumor-bearing mice for 6 MV x-ray irradiation. (C) Representative image of tumor harvesting on day 7. (D) Tumor-growth curves (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. ∗, ∗∗, ∗∗∗, or ∗∗∗∗ represent p-values <0.05, < 0.01, < 0.001, and <0.0001, respectively. X-axis: Number of days, corresponding to the treatment timeline, with the first day being the first day of treatment. Y-axis: Tumor volume growth, measured as the difference between the tumor volume on the day of measurement and the tumor volume before treatment (first day). (E) Body-weight changes over the study period (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. X-axis: Number of days. Y-axis: Mouse weight, measured as mouse weight on the day of measurement.

Journal: Technology in Cancer Research & Treatment

Article Title: Combining Radiation and anti-PD-L1 Enhances the Antitumor Activity in Colorectal Cancer via IFN-γ-Dependent Activation of STAT1

doi: 10.1177/15330338251406931

Figure Lengend Snippet: In vivo treatment schedule and therapeutic outcomes. (A) Experimental timeline for control, radiotherapy (IR), anti-PD-L1 (ICI), and combination groups. (B) Positioning of tumor-bearing mice for 6 MV x-ray irradiation. (C) Representative image of tumor harvesting on day 7. (D) Tumor-growth curves (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. ∗, ∗∗, ∗∗∗, or ∗∗∗∗ represent p-values <0.05, < 0.01, < 0.001, and <0.0001, respectively. X-axis: Number of days, corresponding to the treatment timeline, with the first day being the first day of treatment. Y-axis: Tumor volume growth, measured as the difference between the tumor volume on the day of measurement and the tumor volume before treatment (first day). (E) Body-weight changes over the study period (mean ± SD; n = 4 per group); statistical methods: one-way ANOVA. X-axis: Number of days. Y-axis: Mouse weight, measured as mouse weight on the day of measurement.

Article Snippet: Therapeutic agents: anti-mouse PD-L1 antibody (clone 10F.9G2), recombinant mouse IFN-γ and Itacitinib (both MedChemExpress).

Techniques: In Vivo, Control, Irradiation

STAT1 inhibition assay. (A-B) Western blots and Annexin V/PI staining demonstrated that pharmacologic inhibition of STAT1 by Itacitinib abolished IFN-γ induced caspase-3 cleavage, and apoptosis in CT26.WT cells. Grayscale densitometry for all Western blots has been performed using ImageJ, normalized to β-actin (n = 3 independent experiments). Bars represent mean ± SD. Statistical comparison was performed using an unpaired two-tailed t-test.

Journal: Technology in Cancer Research & Treatment

Article Title: Combining Radiation and anti-PD-L1 Enhances the Antitumor Activity in Colorectal Cancer via IFN-γ-Dependent Activation of STAT1

doi: 10.1177/15330338251406931

Figure Lengend Snippet: STAT1 inhibition assay. (A-B) Western blots and Annexin V/PI staining demonstrated that pharmacologic inhibition of STAT1 by Itacitinib abolished IFN-γ induced caspase-3 cleavage, and apoptosis in CT26.WT cells. Grayscale densitometry for all Western blots has been performed using ImageJ, normalized to β-actin (n = 3 independent experiments). Bars represent mean ± SD. Statistical comparison was performed using an unpaired two-tailed t-test.

Article Snippet: Therapeutic agents: anti-mouse PD-L1 antibody (clone 10F.9G2), recombinant mouse IFN-γ and Itacitinib (both MedChemExpress).

Techniques: Inhibition, Western Blot, Staining, Comparison, Two Tailed Test